Journal: Chinese Medicine

Article Title: Single-cell RNA sequencing reveals the therapeutic mechanism of Calvatia lilacina in promoting wound healing of anal fistula

doi: 10.1186/s13020-025-01293-w

Figure Lengend Snippet: The impact of CLS on inflammation, angiogenesis, and collagen at different time points. A – C ELISA detection of TNF-α, VEGF, and Collagen I expression levels in granulation tissue; D Immunohistochemical staining of CD14 and CD11b; E , F Immunohistochemical quantitative analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns: not significant ( P > 0.05) vs.the control group; n = 10

Article Snippet: TNF-α, COL1A1, VEGF Elisa Kit (EK182, EK183, EK1C01, Multi Sciences, China); WASF3 Antibody (67620-1-lg, Proteintech, China); α-SMA (19245s, CST, USA); CD14 Antibody (ab183322, abcam, Britain); Cluster of Differentiation 11b (CD11b) Antibody (ab133357, abcam, Britain); IL-6 flow Antibody (562050, BD Pharmingen, USA); JAK2 (WL02188, Wanleibio, China); p-JAK2 (WL02997, Wanleibio, China); STAT3 (WL03207, Wanleibio, China); p-STAT3 (WL06214, Wanleibio, China);

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemical staining, Staining, Control

Journal: Chinese Medicine

Article Title: Single-cell RNA sequencing reveals the therapeutic mechanism of Calvatia lilacina in promoting wound healing of anal fistula

doi: 10.1186/s13020-025-01293-w

Figure Lengend Snippet: Flow cytometry and SEM detection of adhesion and effect of CLS on macrophages. A Flow cytometry was used to determine the ratio of IL-6 positive cells in macrophage after extraction. B Quantitative analysis the rate of IL-6 positive macrophage. C , D Elisa detection of IL-6 and CXCL-8 in cell supernatant. E , F Intensity of intercellular communication between control group and treatment group. G Analysis of IL-6 + macrophage and fibroblast receptor-ligand binding. H Analysis of cell population interaction intensity of IL-6, CXCL-8 signaling pathway. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant ( P > 0.05) vs.the control group; n = 3

Article Snippet: TNF-α, COL1A1, VEGF Elisa Kit (EK182, EK183, EK1C01, Multi Sciences, China); WASF3 Antibody (67620-1-lg, Proteintech, China); α-SMA (19245s, CST, USA); CD14 Antibody (ab183322, abcam, Britain); Cluster of Differentiation 11b (CD11b) Antibody (ab133357, abcam, Britain); IL-6 flow Antibody (562050, BD Pharmingen, USA); JAK2 (WL02188, Wanleibio, China); p-JAK2 (WL02997, Wanleibio, China); STAT3 (WL03207, Wanleibio, China); p-STAT3 (WL06214, Wanleibio, China);

Techniques: Flow Cytometry, Extraction, Enzyme-linked Immunosorbent Assay, Control, Ligand Binding Assay

Journal: Nature Communications

Article Title: KY216-tubulin complex captures VASH2 to inhibit NSCLC metastasis

doi: 10.1038/s41467-025-66817-2

Figure Lengend Snippet: a Effects of KY216 on cellular microtubule networks (independent biological replicates n = 3). NCI-H460 cells were exposed to varying concentrations of KY216 for 48 h. Confocal microscopy was used to capture images of microtubules (labeled green with anti-α-tubulin antibody) and nuclei (stained with DAPI and labeled blue). Scale bar: 20 μm. b The formation of NCI-H460, A549, and 95-D cell colonies was significantly affected by different concentrations of KY216 (independant biological replicates n = 3). c Fluorescent expression of EDU+ cells was observed in NCI-H460 and 95-D cells. The EDU+ cells were labeled with green fluorescence, while the cell nuclei were stained with DAPI to show blue fluorescence (independent biological replicates n = 3). Scale bar: 100 μm. The images were acquired using the ImageXpress Micro® system. d , e Flow cytometry reveals that KY216 induces G2/M cycle arrest in NCI-H460 and 95-D cells ( d ), with significance indicated by comparison of the G2/M phase proportion in each group. Corresponding Western blot analysis is shown in ( e ) (independent biological replicates n = 3). f Conditioned medium from NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 inhibited the formation of capillary-like tubular networks in HUVECs. Representative images are shown (independent biological replicates n = 3). Scale bar: 50 μm. g Determination of VEGF expression levels in supernatants of NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 (independent biological replicates n = 3). All quantitative data are shown as means ± SEM. Values shown are P values and were calculated using the one-way ANOVA with Tukey’s comparisons test in GraphPad Prism 9.5.0 ( b – g ). Source data are provided as a Source Data file.

Article Snippet: The secretion of VEGF was measured using the AuthentiKineTM Human VEGF ELISA Kit (KE00216, Proteintech, China) according to the manufacturer’s guidelines.

Techniques: Confocal Microscopy, Labeling, Staining, Expressing, Fluorescence, Flow Cytometry, Comparison, Western Blot

Journal: BMC Psychiatry

Article Title: Decreased serum VEGF levels and their negative correlation with cognitive function in patients with major depressive disorder: a case-control study

doi: 10.1186/s12888-025-07612-7

Figure Lengend Snippet: Comparison of serum VEGF levels between MDD patients and healthy controls. Abbreviations: VEGF, Vascular Endothelial Growth Factor; ANOVA, Analysis of Variance; ANCOVA, Analysis of Covariance; HCs, Healthy Controls

Article Snippet: The Human VEGF ELISA Kit (EK183-96, MULTI SCIENCES, Hangzhou, China) was used to quantitatively measure VEGF protein levels in the serum samples using a Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific, USA).

Techniques: Comparison

Journal: BMC Psychiatry

Article Title: Decreased serum VEGF levels and their negative correlation with cognitive function in patients with major depressive disorder: a case-control study

doi: 10.1186/s12888-025-07612-7

Figure Lengend Snippet: A negative correlation between serum VEGF levels and attention score in MDD patients ( r =-0.32, p = 0.01), but this correlation was not observed in the healthy control group ( r = 0.19, p = 0.15)

Article Snippet: The Human VEGF ELISA Kit (EK183-96, MULTI SCIENCES, Hangzhou, China) was used to quantitatively measure VEGF protein levels in the serum samples using a Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific, USA).

Techniques: Control

Journal: BMC Psychiatry

Article Title: Decreased serum VEGF levels and their negative correlation with cognitive function in patients with major depressive disorder: a case-control study

doi: 10.1186/s12888-025-07612-7

Figure Lengend Snippet: A negative correlation between serum VEGF levels and RBANS total score in MDD patients ( r =-0.28, p = 0.03), but this correlation was not observed in the healthy control group ( r =-0.03, p = 0.82)

Article Snippet: The Human VEGF ELISA Kit (EK183-96, MULTI SCIENCES, Hangzhou, China) was used to quantitatively measure VEGF protein levels in the serum samples using a Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific, USA).

Techniques: Control